Collections / Spatial Transcriptomics of Lungs Infected with Coccidioides posadasii

Spatial Transcriptomics of Lungs Infected with Coccidioides posadasii

Coccidioidomycosis, or Valley Fever, is a lung disease caused by inhaling Coccidioides fungi, prevalent in the Southwestern U.S., Mexico, and parts of Central and South America. Climate change is contributing to the spread of this disease. Many cases are asymptomatic, some are often misdiagnosed, and a small subset can progress to severe illness. To better understand lung responses during late Coccidioides infection, we used 10x Visium spatial transcriptomics. We analyzed non-infected lung (D0) and infected lung at 14 days post-infection (D14).

Datasets2
Size~64 MB
AssayVisium
Samplemouse · lung
KindTime course
PMID:39611685 ↗
Python · lamindb
import lamindb as ln
import spatialdata as sd

coll = ln.Collection.get(key="geo-gse274767")
coll.ordered_artifacts.df()                 # the 2 members, ordered

# download every member .zarr to the local cache:
paths = [a.cache() for a in coll.ordered_artifacts.all()]

# …or load each straight into memory as a SpatialData object:
sdatas = {a.key: sd.read_zarr(a.cache()) for a in coll.ordered_artifacts.all()}
Open in Vitessce
Visium
Mouse lung
GSM8457567
mouse lung coccidioidomycosis 26 MB 4,991 obs bronze H&E
TechnologyVisium
Organismmouse
Tissuelung
Conditioncoccidioidomycosis
Size26 MB
Obs4,991
Magnificationother · 5.450 µm/px
bronze H&E Open in Vitessce
Open in Vitessce
Visium
Mouse lung
GSM8457566
mouse lung 37 MB 4,936 obs bronze H&E
TechnologyVisium
Organismmouse
Tissuelung
Size37 MB
Obs4,936
Magnificationother · 5.485 µm/px
bronze H&E Open in Vitessce

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