Collections / Spatial Transcriptomics reveals that cells that experience intratumoral hypoxia retain hypoxia-associated transcriptional programs after invading into oxygenated tumor regions.

Spatial Transcriptomics reveals that cells that experience intratumoral hypoxia retain hypoxia-associated transcriptional programs after invading into oxygenated tumor regions.

To understand the role of hypoxia in metastatic progression, we sought to spatially map gene expression in hypoxic cells compared with cells that migrated to oxygenated tumor regions. We adapted the Visium spatial transcriptomics protocol to perform concurrent transcriptional profiling and fluorescent imaging of our hypoxia fate-mapping system.

Datasets2
Size~89 MB
AssayVisium
Samplehuman · breast
KindReplicates
PMID:39341835 ↗
Python · lamindb
import lamindb as ln
import spatialdata as sd

coll = ln.Collection.get(key="geo-gse240212")
coll.ordered_artifacts.df()                 # the 2 members, ordered

# download every member .zarr to the local cache:
paths = [a.cache() for a in coll.ordered_artifacts.all()]

# …or load each straight into memory as a SpatialData object:
sdatas = {a.key: sd.read_zarr(a.cache()) for a in coll.ordered_artifacts.all()}
Open in Vitessce
Visium
Human breast
GSM7688225
human breast breast cancer 42 MB 2,846 obs bronze H&E
TechnologyVisium
Organismhuman
Tissuebreast
Conditionbreast cancer
Size42 MB
Obs2,846
Magnificationother · 3.380 µm/px
bronze H&E Open in Vitessce
Open in Vitessce
Visium
Human breast
GSM7688224
human breast breast cancer 46 MB 2,536 obs bronze H&E
TechnologyVisium
Organismhuman
Tissuebreast
Conditionbreast cancer
Size46 MB
Obs2,536
bronze H&E Open in Vitessce

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