Collections / ETMR stem-like state and chemo-resistance are supported by perivascular cells at single-cell resolution (spatial transcriptomics subset)

ETMR stem-like state and chemo-resistance are supported by perivascular cells at single-cell resolution (spatial transcriptomics subset)

To investigate the celluar heterogeneity of ETMR and the role of pericytes in the tumor microenvironment, we performed single-cell RNA sequencing of about 140,000 cells from primary human and murine ETMR as well as from 2D ETMR cultures and 3D models of ETMR-harboring forebrain organoids. We also analysed 3 ETMR human samples by spatial transcriptomics.

Datasets3
Size~113 MB
AssayVisium
Samplehuman · brain
KindOther
PMID:40562749 ↗
Python · lamindb
import lamindb as ln
import spatialdata as sd

coll = ln.Collection.get(key="geo-gse224477")
coll.ordered_artifacts.df()                 # the 3 members, ordered

# download every member .zarr to the local cache:
paths = [a.cache() for a in coll.ordered_artifacts.all()]

# …or load each straight into memory as a SpatialData object:
sdatas = {a.key: sd.read_zarr(a.cache()) for a in coll.ordered_artifacts.all()}
Open in Vitessce
Visium
Human brain
GSM7024964
human brain embryonal tumor with multilayered rosettes 21 MB 848 obs bronze H&E
TechnologyVisium
Organismhuman
Tissuebrain
Conditionembryonal tumor with multilayered rosettes
Size21 MB
Obs848
bronze H&E Open in Vitessce
Open in Vitessce
Visium
Human brain
GSM7024966
human brain embryonal tumor with multilayered rosettes 36 MB 2,668 obs bronze H&E
TechnologyVisium
Organismhuman
Tissuebrain
Conditionembryonal tumor with multilayered rosettes
Size36 MB
Obs2,668
bronze H&E Open in Vitessce
Open in Vitessce
Visium
Human brain
GSM7024965
human brain embryonal tumor with multilayered rosettes 56 MB 3,814 obs bronze H&E
TechnologyVisium
Organismhuman
Tissuebrain
Conditionembryonal tumor with multilayered rosettes
Size56 MB
Obs3,814
bronze H&E Open in Vitessce

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