import lamindb as ln
import spatialdata as sd
coll = ln.Collection.get(key="doi-10.1038/s41467-023-40314-w")
coll.ordered_artifacts.df() # the 4 members, ordered
# download every member .zarr to the local cache:
paths = [a.cache() for a in coll.ordered_artifacts.all()]
# …or load each straight into memory as a SpatialData object:
sdatas = {a.key: sd.read_zarr(a.cache()) for a in coll.ordered_artifacts.all()}Neuropathy is a feature more frequently observed in pancreatic ductal adenocarcinoma (PDAC) than other tumors. Schwann cells, the most prevalent cell type in peripheral nerves, migrate toward tumor cells and associate with poor prognosis in PDAC. To unveil the effects of Schwann cells on the neuro-stroma niche, here we perform single-cell RNA-sequencing and microarray-based spatial transcriptome analysis of PDAC tissues. Results suggest that Schwann cells may drive tumor cells and cancer-associated fibroblasts (CAFs) to more malignant subtypes: basal-like and inflammatory CAFs (iCAFs), respectively. Moreover, in vitro and in vivo assays demonstrate that Schwann cells enhance the proliferation and migration of PDAC cells via Midkine signaling and promote the switch of CAFs to iCAFs via interleukin-1α. Culture of tumor cells and CAFs with Schwann cells conditioned medium accelerates PDAC progression. Thus, we reveal that Schwann cells induce malignant subtypes of tumor cells and CAFs in the PDAC milieu.
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Visium @article{Xue_2023,
title = {Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment},
volume = {14},
ISSN = {2041-1723},
url = {http://dx.doi.org/10.1038/s41467-023-40314-w},
DOI = {10.1038/s41467-023-40314-w},
number = {1},
journal = {Nature Communications},
publisher = {Springer Science and Business Media LLC},
author = {Xue, Meilin and Zhu, Youwei and Jiang, Yongsheng and Han, Lijie and Shi, Minmin and Su, Rui and Wang, Liwen and Xiong, Cheng and Wang, Chaofu and Wang, Ting and Deng, Shijie and Wu, Dong and Cao, Yizhi and Dong, Lei and Bai, Fan and Zhao, Shulin and Deng, Xiaxing and Peng, Chenghong and Li, Hongwei and Chen, Jianjun and Shen, Baiyong and Jiang, Lingxi and Chen, Hao},
year = {2023},
month = July
}